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Image Search Results
Journal: Fems Microbiology Letters
Article Title: Regulation of Cu(I)/Ag(I) efflux genes in Escherichia coli by the sensor kinase CusS
doi: 10.1111/j.1574-6968.2012.02529.x
Figure Lengend Snippet: Data shows the relative expression from the cusC gene +/− SEM, as determined in E. coli wild-type (gray), E. coli ΔcusS (checkered) and E. coli ΔcusS/pBADcusS (diagonal lines) after exposure to 5 µM AgNO3 for 0, 2 and 4 hours. Total RNA from cells was extracted, reverse transcribed and cDNA was subjected to real-time PCR using primers specific for cusC. ANOVA analysis with multiple comparisons showed that transcription from cusC is absent when cusS is disrupted (P < 0.0001).
Article Snippet: First strand cDNA was prepared from 2 µg of total RNA using the
Techniques: Expressing, Real-time Polymerase Chain Reaction